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Fluka Chemical ssc buffer solution
Ssc Buffer Solution, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ssc+buffer/buffer+solution+ssc/pm42076203-45-11-20
Average 86 stars, based on 1 article reviews
ssc buffer solution - by Bioz Stars, 2026-10
86/100 stars

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Injection:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.

Incubation:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.

Hybridization:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.

Concentration Assay:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.

Labeling:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.

Saline:

Article Title: Amplified plasmonic detection of DNA hybridization using doxorubicin-capped gold particles.
Article Snippet: We show in this article that doxorubicin-modified gold nanoparticles (Au NP-DOX) can be used for the post-amplification of the wavelength shift of localized surface plasmon resonance (LSPR) signals after DNA hybridization events.. We take advantage of the intercalation properties of DOX with guanine-rich oligonucleotides and the plasmon coupling between surface-linked gold nanostructures and Au NP-DOX in solution to detect in a sensitive manner DNA hybridisation events.. Post-treatment of double-stranded DNA with Au NP-DOX resulted in a detection limit of z600 pM, several times lower than that without post-incubation (LOD z 40 nM).

Article Title: Approach for plasmonic based DNA sensing: amplification of the wavelength shift and simultaneous detection of the plasmon modes of gold nanostructures.
Article Snippet: In this article, the detection of DNA hybridization taking advantage of the plasmonic properties of gold nanostructures is described.. The approach is based on the amplification of the wavelength shift of a multilayered localized surface plasmon resonance (LSPR) sensor interface upon hybridization with gold nanorods and nanostarslabeled DNA.. The amplification results in a significant decrease of the limit of detection from ≈40 nM as observed for unlabeled DNA to 0.2 nM for labeled DNA molecules.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR-ABL Gene Sequence Detection Using a Novel Gold Nanoparticle-Enhanced Sandwich Assay Format.
Article Snippet: The reagents used in this research were as follows: urea, KH2PO4, SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Development of a Highly Sensitive SPR Biosensor for BCR–ABL Gene Sequence Detection Using a Novel Gold Nanoparticle–Enhanced Sandwich Assay Format
Article Snippet: The reagents used in this research were as follows: urea, KH 2 PO 4 , SSC buffer solution, lipoic acid (LA) and 6-mercapto-1-hexanol (MCH) (Fluka, Buchs, Switzerland), oligonucleotides (Metabion, Planegg, Germany), aurum (III) chloride, and dilute HCL (Thermo Fisher Scientific, Waltham, MA, USA); all reagents were of Pro Analysi quality.

Article Title: Ternary DNA chip based on a novel thymine spacer group chemistry.
Article Snippet: A novel thymine-based surface chemistry suitable for label-free electrochemical DNA detection is described.. It involves a simple two-step sequential process: immobilization of 9-mer thymine-terminated probe DNAs followed by backfilling with 9-mer thymine-based spacers (T9).. As compared to commonly used organic spacer groups like 2-mercaptoethanol, 3-mercapto-1-propanol and 6-mercapto-1-hexanol, the 9-mer thymine-based spacers offer a 10-fold improvement in discriminating between complementary and non-complementary target hybridization, which is due mainly to facilitated transport of the redox probes through the probe-DNA/T9 layers.

Article Title: Development and optimization of an 18S rRNA-based oligonucleotide microarray for the fungal order Eurotiales.
Article Snippet: The oligonucleotides were diluted in 3· saline sodium citrate buffer (SSC) (Fluka, Buchs, Switzerland), SSC-based Nexterion Spot LE (SLE) buffer and 50% DMSO (dimethyl sulfoxide) (SigmaAldrich, Germany) to a final concentration of 25 lmol l)1 and spotted in duplicate onto Nexterion Slide E (epoxy coated) using a Microgrid II TAS (Biorobotics, Cambridge, UK) spotter.

Article Title: Downregulation of miR‐326 and its host gene β‐arrestin1 induces pro‐survival activity of E2F1 and promotes medulloblastoma growth
Article Snippet: Prehybridization was performed using 50% formamide (Sigma‐Aldrich F9037), 5× saline‐sodium citrate (SSC) buffer (FLUKA cat. S6639‐1L), 0.1% Tween‐20, 9.2 m m Citric Acid (Sigma‐Aldrich C1909), 50 μg·mL −1 heparin (Sigma H4784), and 500 μg·mL −1 Yeast RNA (Sigma‐Aldrich R6750) for 3 h at 62 °C.



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